Study of total phenol, flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown Thymus vulgaris
10.1016/S2221-1691(13)60142-2
- Author:
Hossain Amzad Mohammad
;
AL-Mijizy Hamood Zawan
;
Weli Mohammed Afaf
;
Al-Riyami Qasim
- Publication Type:Journal Article
- Keywords:
Thymus vulgaris L.;
Various crude extracts;
Phytochemical screening;
Soxhlet extractor;
Total phenol;
Flavonoids contents
- From:Asian Pacific Journal of Tropical Biomedicine
2013;(9):705-710
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare various crude extracts using different polarities of solvent and to quantitatively evaluate their total phenol, flavonoids contents and phytochemical screening of Thymus vulgaris collected from Al Jabal Al Akhdar, Nizwa, Sultanate of Oman.
Methods:The leave sample was extracted with methanol and evaporated. Then it was defatted with water and extracted with different polarities organic solvents with increasing polarities. The prepare hexane, chloroform, ethyl acetate, butanol and methanol crude extracts were used for their evaluation of total phenol, flavonoids contents and phytochemical screening study. The established conventional methods were used for quantitative determination of total phenol, flavonoids contents and phytochemical screening.
Results: Phytochemical screening for various crude extracts were tested and shown positive result for flavonoids, saponins and steroids compounds. The result for total phenol content was the highest in butanol and the lowest in methanol crude extract whereas the total flavonoids contents was the highest in methanol and the lowest hexane crude extract.
Conclusions:The crude extracts from locally grown Thymus vulgaris showed high concentration of flavonoids and it could be used as antibiotics for different curable and uncurable diseases.
