Simultaneous HPTLC-UV530 nm analysis and validation of bioactive lupeol and stigmasterol in Hygrophila auriculata (K. Schum) Heine
- Author:
Hussain Sarfaraj Md
;
Fareed Sheeba
;
Ali Mohammad
- Publication Type:Journal Article
- Keywords:
Hygrophila auriculata;
HPTLC-UV;
Lupeol;
Stigmasterol;
Validation
- From:Asian Pacific Journal of Tropical Biomedicine
2012;(z2):612-617
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyse the two marker compounds lupeol (LP) and stigmasterol (ST) from methanolic extract of Hygrophila auriculata (H. auriculata). Methods: Separation was achieved on aluminium plates precoated with silica gel 60F254 with toluene-methanol-formic acid (7.0:2.7: 0.3 v/v/v) as mobile phase. Results: Densitometric analysis was performed at 530 nm in the reflectance mode. Compact bands for LP and ST were obtained at RF 0.52 ± 0.02 and 0.28 ± 0.05. Linearity (r2=0.998 5 and 0.993 7), limit of detection (45 and 18 ng/band) limit of quantification (135 and 54 ng/band), recovery (98.2%-99.7% and 97.2%-99.6%), and precision (<2.18 and 1.91) were satisfactory for LP and ST respectively. Linearity range for LP and ST were 100-1 000 and 50-500 ng/band and the contents estimated as (0.19±0.1)% and (0.47±0.1)% w/w respectively. Conclusion:The method demonstrated efficient analysis testing of LP and ST in samples; therefore it can be used for routine analysis.
