Enhancement of erectile function of sexually na?ve rats by β-sitosterol and α-β-amyrin acetate isolated from the hexane extract of Mondiawhitei
- Author:
Watcho Pierre
;
Zelefack Fabien
;
Ngouela Silvere
;
Nguelefack Benot Telesphore
;
Kamtchouing Pierre
;
Tsamo Etienne
;
Kamanyi Albert
- Publication Type:Journal Article
- Keywords:
Mondia whitei;
β-sitosterol;
α and β-amyrin acetate;
aphrodisiac;
rat
- From:Asian Pacific Journal of Tropical Biomedicine
2012;(z3):1266-1269
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The aim of the study was to investigate the prosexual activity of two compounds isolated from the hexane extract of the dried roots of Mondia whitei in rats. Methods: β-sitosterol and the mixture of α and β-amyrin acetate were isolated and characterised using several reagents, chromatography, mass spectrometry and physical analysis (RMN, 1H and 13C). Sexually inexperienced adult male rats were orally treated with these compounds at doses of 0mg/kg (control), 10mg/kg or 50mg/kg. 1h after the treatment, mount and intromission latencies and frequencies, penile erection, ejaculation latency and post-ejaculatory interval were measured for 60 minutes. Results: β-sitosterol and the mixture of α and β-amyrin acetate significantly increased the mount frequency (p<0.05), penile erection (P<0.001) and the ejaculation latency when compared to control. The intromission frequency remained unchanged (P>0.05) when compared to control. The two purified compounds were efficient at the lower dose (10 mg/kg b.w) with β-sitosterol being the most potent. Conclusion:Results of the present work give added value to the aphrodisiac property of Mondia whitei and further justify its popular use as a sex stimulant.
