Biocompatibility of folate-modified chitosan nanoparticles
10.1016/S2221-1691(12)60044-6
- Author:
Chakraborty Prasad Subhankari
;
Sahu Kumar Sumanta
;
Pramanik Panchanan
;
Roy Somenath
- Publication Type:Journal Article
- Keywords:
Carboxymethyl chitosan;
Folate;
Staphylococcus aureus;
Killing;
kinetic studies;
MTT assay
- From:Asian Pacific Journal of Tropical Biomedicine
2012;(3):215-219
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the acute toxicity of carboxymethyl chitosan-2, 2’ ethylenedioxy bis-ethylamine-folate (CMC-EDBE-FA) and as well as possible effect on microbial growth and in vitro cell cyto-toxicity. Methods: CMC-EDBE-FA was prepared on basis of carboxymethyl chitosan tagged with folic acid by covalently linkage through 2, 2’ ethylenedioxy bis-ethylamine. In vivo acute toxicity, in vitro cyto-toxicity and antimicrobial activity of CMC-EDBE-FA nanoparticle were determined. Results: Vancomycin exhibited the antibacterial activity against vancomycin sensitive Staphylococcus aureus, but CMC-EDBE-FA nanoparticle did not give any antibacterial activity as evidenced by minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), disc agar diffusion (DAD) and killing kinetic assay. Further, the CMC-EDBE-FA nanoparticle showed no signs of in vivo acute toxicity up to a dose level of 1000 mg/kg p.o., and as well as in vitro cyto-toxicity up to 250 μg/mL. Conclusions: These findings suggest that CMC-EDBE-FA nanoparticle is expected to be safe for biomedical applications.
