Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production
- Author:
Hilario Felipe Fabrine
;
Nascimento Joventino Gabriel
- Publication Type:Journal Article
- Keywords:
Sisal;
Cell culture;
Mosquito;
Insecticide
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(5):396-399
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of Agave sisalana (A. sisalana) extract on Aedes aegypti (Ae. aegypti) primary cell culture.
Methods: Cells of Ae. aegypti were exposed to different concentrations of A. sisalana crude extract (0.18–6.00 mg/mL) for 24 h. Then, the cells were labeled with propidium iodide and subjected to fluorescence microscopy to verify cell viability. In addition, nitric oxide production was measured.
Results: Results showed that cells exposed to 6 mg/mL of the crude extract presented a greater percentage of death when compared to control (73.8%± 9.6%vs. 34.6%± 9.6%). Furthermore, there was an increase in the nitric oxide production in cells exposed to 6 mg/mL of A. sisalana crude extract [(0.81 ± 0.08) mmol/L] compared to control group [(0.41 ± 0.18) mmol/L].
Conclusions: The results show that A. sisalana is cytotoxic to Ae. aegypti and may be used as raw material for new eco-friendly and inexpensive insecticides, since sisal in-dustry discards the liquid waste for the extraction of plant fiber.
