Analysis of human B cell response to recombinantLeishmaniaLPG3
- Author:
Fatahaliha Haji Mostafa
;
Hosseini Maryam
;
Rasolzadeh Sanaz
;
Bandi Shane Dariush
;
Baradaran Behzad
;
Jadidi-Niaragh Farhad
;
Yousefi Mehdi
- Publication Type:Journal Article
- Keywords:
Leishmania;
LPG3;
Vaccine;
Cytokine;
Bcell;
CD69
- From:Asian Pacific Journal of Tropical Biomedicine
2015;(8):615-619
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the capability of recombinant Leishmania LPG3 and its fragments in the activation of B cells.Methods:In the present study, human B cells were purified from peripheral blood of 10 adult healthy subjects using magnetic-activated cell sorting technique. Subsequently, purified B cells were treated with recombinant LPG3, and itsN-terminal and C-terminal fragments at different concentrations (2, 10 and 20 μg/mL). B cell activation was assessed through expression of CD69 molecule by flowcytometry and secretion of IL-6, TNF-αα and IL-10 cytokines via enzyme-linked immunosorbent assay following treatment with recombinant antigens.Results:Our results showed that while the recombinant LPG-3 could significantly increase the production of IL-6 and TNF-α (P<0.05) in B cells, it had no effect on the secretion of IL-10 by B cells.Conclusions: Our study indicated that recombinant LPG-3 and especially itsN-terminal fragment could stimulate B cell response as an important immune response component against leishmaniasis. Thus, it seems that it can be considered as an effective adjuvant in vaccine developments against leishmaniasis.
