Quantification of tolvaptan in rabbit plasma by LC-MS/MS:Application to a pharmacokinetic study
10.1016/j.jpha.2014.09.001
- Author:
Moola S. Kumar
;
Bannoth Kothapalli Chandrasekhar
- Publication Type:Journal Article
- Keywords:
Tolvaptan;
LC-ESI-MS/MS;
Rabbit plasma;
Liquid-liquid extraction;
Pharmacokinetics
- From:
Journal of Pharmaceutical Analysis
2015;(6):371-377
- CountryChina
- Language:Chinese
-
Abstract:
A sensitive, selective and high-throughput liquid chromatography–tandem mass spectrometry (LC–ESI–MS/MS) method was developed and validated for the quantitation of tolvaptan in rabbit plasma. Sample clean-up involved liquid–liquid extraction (LLE) and chromatography was performed on Zorbax SB C18 analytical column (50 mm ? 2.1 mm, 3.5 mm) using 0.1%formic acid:methanol (20:80, v/v) as the mobile phase. The parent-product ion transitions for the drug (m/z 449.2-252.1) and IS (m/z 456.2-259.2) were monitored on a triple quadrupole mass spectrometer, operating in the multiple reaction monitoring (MRM) and positive ion mode. The method was validated over the concentration range of 0.10–1000.00 ng/mL and successfully applied to a pharmacokinetic study of healthy rabbits.
