The acquisition of drug resistance plasmid in Pseudomonas aeruginosa induced by quinolones
10.3969/j.issn.1673-4130.2015.17.006
- VernacularTitle:用喹诺酮诱导铜绿假单胞菌获得耐药质粒的研究
- Author:
Shike LUO
;
Xianhua LIU
;
He WU
;
Xian JIN
;
Chunhui FAN
;
Ping′an ZHU
;
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
quinolones;
mutation;
plasmid;
drug resistance
- From:
International Journal of Laboratory Medicine
2015;(17):2469-2470
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveToinvestigatetheplasmidgenechangesinquinolone‐sensitivePseudomonasaeruginosa.Methods 31iso‐lates from January 2011 to December 2013 from various qualified clinical samples in the hospital were collected .In the 31 isolateds , 16 isolates proved sensitive to quinolones by using K‐B method were used as research objects in the study .The isolates growing ou‐side the sensitive ring of ciprofloxacin paper were selected to continuously transferred into other culture dishes until the resistance to quinolones were acquired .Plasmid transformation and extraction were performed on those isolates to confirm the existence of drug‐resistanceplasmidsacquired,andthroughPCRandgenesequenceanalysistodeterminethetypeofplasmids.Results 2iso‐lates of quinolone‐sensitive Pseudomonas aeruginosa acquired drug‐resistance plasmids qnrS and were resistant quinolones induced by continuous transferring for 9 times .Conclusion If antibiotics of inhibitory concentration were often used for the treatment of Pseudomonas aeruginosa infection ,drug‐resistance plasmids were acquired easily .
