Rapid screening and distribution of bioactive compounds in different parts of Berberis petiolaris using direct analysis in real time mass spectrometry
10.1016/j.jpha.2015.05.002
- Author:
Singh Awantika
;
Bajpai Vikas
;
Srivastava Mukesh
;
Arya Ram Kamal
;
Kumar Brijesh
- Publication Type:Journal Article
- Keywords:
Berberis petiolaris;
Alkaloids;
Profiling;
DART-TOF-MS;
Statistical analysis
- From:
Journal of Pharmaceutical Analysis
2015;5(5):332-335
- CountryChina
- Language:Chinese
-
Abstract:
Berberis petiolaris Wall. ex G. Don, an unexplored medicinal plant belonging to the family Berberidaceae, is a large deciduous shrub found in Western Himalaya between 1800-3000 m. Chemical profiling of fruit, leaf, root and stem was done by direct analysis in real time mass spectrometry followed by multivariate analysis for discrimination among the plant parts. The bioactive compounds, including magnoflorine, berberine, jatrorrhizine, thalifendine/berberrubine, demethyleneberberine, reticuline, 8-oxoberberine, N-methyltetrahydroberberine, tetrahydropalmatine, tetrahydroberberine and palmatine, were identified by their exact mass measurement and the corresponding molecular formula of each compound. A comparative study of distribution pattern for all these bioactive alkaloids showed qualitative and quantitative variations in different parts of B. petiolaris. Principal component analysis clearly dis-criminated each part of B. petiolaris plant.
