Determination of cilostazol and its active metabolite 3,4-dehydro cilostazol from small plasma volume by UPLC-MS/MS
10.1016/j.jpha.2014.08.001
- Author:
Bhatt M. Nejal
;
Chavada D. Vijay
;
Patel P. Daxesh
;
Sharma Primal
;
Sanyal Mallika
;
Shrivastav S. Pranav
- Publication Type:Journal Article
- Keywords:
Cilostazol;
Sensitive;
High throughput 3,4-dehydro cilostazol;
UPLC-MS/MS
- From:
Journal of Pharmaceutical Analysis
2015;(1):1-11
- CountryChina
- Language:Chinese
-
Abstract:
A simple, rapid and sensitive ultra performance liquid chromatography-tandem mass cilostazol and its pharmacologically active metabolite 3,4-dehydro cilostazol in human plasma using deuterated analogs as internal standards (ISs). Plasma samples were prepared using solid phase extraction 18 (50 mm ? 2.1 mm, 1.7 mm) column. The method was established over a concentration range of 0.5–1000 ng/mL for cilostazol and 0.5–mL for 3,4-dehydro cilostazol. Intra-and inter-batch precision (%CV) and accuracy for the analytes were found within 0.93–1.88 and 98.8–101.7% for cilostazol and 0.91–2.79 and 98.0–102.7% for the metabolite respectively. The assay recovery was within 95–97% for both the analytes and internal standards. The method was successfully applied to support a bioequivalence study of 100 mg cilostazol in 30 healthy subjects.
