Analytical quality-by-design approach for sample treatment of BSA-containing solutions
10.1016/j.jpha.2014.06.001
- Author:
Taevernier Lien
;
Wynendaele Evelien
;
D’Hondt Matthias
;
Spiegeleer De Bart
- Publication Type:Journal Article
- Keywords:
Bovine serum albumin (BSA) solutions;
Franz diffusion cell (FDC);
Analytical quality-by-design (QbD);
Sample preparation;
Design of experiment (DOE);
Derringer desirability (D)
- From:
Journal of Pharmaceutical Analysis
2015;(1):27-32
- CountryChina
- Language:Chinese
-
Abstract:
The sample preparation of samples containing bovine serum albumin (BSA), e.g., as used in transdermal Franz diffusion cell (FDC) solutions, was evaluated using an analytical quality-by-design (QbD) approach. Traditional precipitation of BSA by adding an equal volume of organic solvent, often successfully used with conventional HPLC-PDA, was found insufficiently robust when novel fused-core HPLC and/or UPLC-MS methods were used. In this study, three factors (acetonitrile (%), formic acid (%) and boiling time (min)) were included in the experimental design to determine an optimal and more suitable sample treatment of BSA-containing FDC solutions. Using a QbD and Derringer desirability (D) approach, combining BSA loss, dilution factor and variability, we constructed an optimal working space with the edge of failure defined as Do0.9. The design space is modelled and is confirmed to have an ACN range of 8373%and FA content of 170.25%.
