Reagent-free determination of amikacin content in amikacin sulfate injections by FTIR derivative spectroscopy in a continuous flow system
- Author:
Ovalles F. José
;
Gallignani Máximo
;
Brunetto R. María
;
Rondón A. Rebeca
;
Ayala Carlos
- Publication Type:Journal Article
- Keywords:
Amikacin;
FTIR derivative spectro-metry;
Continuous flow system;
Pharmaceutical preparation;
Injection;
Sulfate
- From:
Journal of Pharmaceutical Analysis
2014;(2):125-131
- CountryChina
- Language:Chinese
-
Abstract:
The quantitative estimation of amikacin (AMK) in AMK sulfate injection samples is reported using FTIR-derivative spectrometric method in a continuous flow system. Fourier transform of mid-IR spectra were recorded without any sample pretreatment. A good linear calibration (r40.999, %RSDo 2.0) in the range of 7.7-77.0 mg/mL was found. The results showed a good correlation with the manufacturer's and overall they all fell within acceptable limits of most pharmacopoeial monographs on AMK sulfate.
