Identification, synthesis and characterization of an unknown process related impurity in eslicarbazepine acetate active pharmaceutical ingredient by LC/ESI-IT/MS, 1H, 13C and 1H-1H COSY NMR
10.1016/j.jpha.2013.08.004
- Author:
Thomas Saji
;
Paul Kumar Saroj
;
Joshi Chandra Subhash
;
Kumar Vineet
;
Agarwal Ashutosh
;
Vir Dharam
- Publication Type:Journal Article
- Keywords:
Eslicarbazepine acetate;
Characterization;
LC/ESI-IT/MS;
NMR;
Impurity
- From:
Journal of Pharmaceutical Analysis
2014;(5):339-344
- CountryChina
- Language:Chinese
-
Abstract:
A new impurity was detected during high performance liquid chromatographic (HPLC) analysis of eslicarbazepine acetate active pharmaceutical ingredient. The structure of unknown impurity was postulated based on liquid chromatography mass spectrometry using electrospray ionization and ion trap analyzer (LC/ESI-IT/MS) analysis. Proposed structure of impurity was unambiguously confirmed by synthesis followed by characterization using 1H, 13C nuclear magnetic resonance spectrometry (NMR), 1H-1H correlation spectro-scopy (COSY) and infrared spectroscopy (IR). Based on the spectroscopic and spectrometric data, unknown impurity was characterized as 5-carbamoyl-10,11-dihydro-5H-dibenzo[b,f]azepin-10-yl propionate.
