Development and validation of analytical method for the estimation of lamivudine in rabbit plasma
10.1016/j.jpha.2011.08.001
- Author:
Singh Vikram Akhilesh
;
Nath K. Lila
;
Pani R. Nihar
- Publication Type:Journal Article
- Keywords:
Lamivudine;
Rabbit plasma;
Pharmacokinetics;
RP-HPLC
- From:
Journal of Pharmaceutical Analysis
2011;01(4):251-257
- CountryChina
- Language:Chinese
-
Abstract:
Lamivudine has been widely used in the treatment of HIV disease. A reliable, sensitive reversed phase high performance liquid chromatography (RP-HPLC) method was developed and validated for lamivudine in rabbit plasma. The method was developed on Hypersil BDS C-18 column (250 mm × 4.6 mm, 5 μm) using a mobile phase of 0.25％ Triethylamine buffer (pH 3.0):acetonitrile (70:30, v/v). The efficient was monitored by UV detector at 256 nm. The total run time was 15 min with a flow rate of 1.0 mL/min. Calibration curve was linear over the concentration range of 25-2000 ng/mL. The retention times of lamivudine and internal standard (Nelfinavir) were 8.78 min and 10.86 min, respectively. The developed RP-HPLC method can be successfully applied for the quantitative pharmacokinetic parameters determination of lamivudine in rabbit model.
