Clinical analysis on the mobilization and collection of peripheral hematopoietic stem cells in 198 healthy donors
10.3760/cma.j.issn.1009-9921.2011.09.004
- VernacularTitle:供者外周血干细胞动员采集198例临床分析
- Author:
Si-yong HUANG
;
Li LIU
;
Miao-wang HA0
;
Hua HE
;
Zhen GU
;
Renan CHEN
;
Wei-wei QIN
;
Yan-lan WU
;
Guo-hui LI
;
Dan-dan YIN
;
Ying-min LIANG
- Publication Type:Journal Article
- Keywords:
Hematopoietic stem cell transplantation;
Hematopoietic stem cell mobilization;
Collection
- From:
Journal of Leukemia & Lymphoma
2011;20(9):522-524
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the methods and effects on the mobilization and collection of peripheral hematopoietic stem cells.MethodsPeripheral hematopoietic stem cells of 198 cases of healthy donors were selected and mobilized by subcutaneous recombinant human granulocyte colony stimulating factor (rhG-CSF) by (5-10) μg·kg-1 ·d-1 and collected on the 5th day. The effects of gender, height, age and WBC count of donors on mobilization and collection were analyzed. ResultsAll the peripheral hematopoietic stem cells of donors were successfully mobilized.The average counts of mononuclear cells(MNC)and CD34+ cells were (4.19±1.96)×108/kg and (2.98±1.40)×106/kg, respectively, which had no correlation with gender, height and age.The counts of MNC and CD34+ cells collected were positively correlated with the WBC count of peripheral blood (r =0.9201, P =0.0035; r =0.8420, P =0.0149). The donors with WBC ≥20.0×109/L had moresignificant effect than thoseWBC＜20.0×109/L (F =4.688, P =0.0013; F =4.622, P =0.0006). Conclusion The WBC count of peripheral blood from healthy donors is a simple and feasible indicator to predict the quantity of CD34+ cells.
