Estabilishment of automated particle-enhanced turbidimetric immunoassay for the measurement of human and evaluation on methodology
10.3969/j.issn.1673-4130.2015.12.031
- VernacularTitle:颗粒增强透射比浊法测定胱抑素C方法学评价
- Author:
Xinxin WANG
;
Meijun CHEN
;
- Publication Type:Journal Article
- Keywords:
cystatin C;
particle-enhanced turbidimetric immunoassay;
automated biochemistry analyzer
- From:
International Journal of Laboratory Medicine
2015;(12):1710-1712
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate methodologically particle‐enhanced turbidimetric immunoassay (PETIA ) for determining quantitatively Cystatin C .Methods Cystatin C was determined quantitatively with PETIA by Cobas 8000 automatic biochemical analyzer ,and imprecision ,veracity ,sensitivity ,linearity range of the method were investigated .The correlation of fresh serum sam‐ple results between PENIA and PETIA was analyzed .The reference interval was verified .Results The within‐run CV imprecision of samples with low and high concentration of adiponectin was 3 .67% and 1 .15% respectively ,the between‐run CV imprecision of samples with low and high concectration of adiponectin was 4 .08% and 1 .53% respectively .The accuracy of the measurement devi‐ation was only -1 .25% .The sensitivity was 0 .07 mg/L .When the adiponectin concentration was between 0 .2 -8 .0 mg/L ,the method showed good linearity .A strong correlation between PETIA and PENIA was confirmed (Y = 0 .945 8X+ 0 .048 6 ,r2 =0 .991 3 ,r=0 .995 6) .Among 40 cases of healthy physical examination sample ,2 cases were with cystatin C level out of the refer‐ence range supplied by reagent kit .The values that were more than 90% in validation interval .Conclusion The PETIA assay is a convenient ,rapid ,cheap ,accurate and reliable method for quantitating the human serum concentration of adiponectin .This assay is suitable for clinical routine analysis .
