Simultaneous Determination of Echinacoside, Acteoside and Isoacteoside in Total Cistanchis glycosides Capsules by HPLC

VernacularTitle:HPLC法同时测定苁蓉总苷胶囊中松果菊苷、毛蕊花糖苷、异毛蕊花糖苷的含量*
Author: Sude YANG ; Junhua HU ; Jiachun LI ; Zhongkun XU ; Yu'an BI ; Wei XIAO ;
Publication Type:Journal Article
Keywords: HPLC; Total Cistanchis glycosides Capsules; echinacoside; acteoside; isoacteoside; content determination
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2015;17(3):609-613
CountryChina
Language:Chinese
Abstract: This study was aimed to establish an HPLC method for simultaneous determination of the content of echinacoside, acteoside and isoacteoside in Total Cistanchis glycosides Capsules. Waters C18 column (4.6 mm ×150 mm, 5 μm) was used with the mobile phase of methanol-0.1% formic acid at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 330 nm. The column temperature was maintained at 30℃ . The results showed that the linear ranges of echinacoside, acteoside and isoacteoside were in the range of 27.792-277.92 μg·mL-1 (r = 0.9996,n = 6), 2.4184-24.184 μg·mL-1 (r = 0.9996, n = 6), 5.106-51.06 μg·mL-1 (r = 0.9998, n = 6). The average recoveries of three components were in accordance with the determination requirement. It was concluded that the method was simple and accurate, which can be used in the content determination of echinacoside, acteoside and isoacteoside in Total Cistanchis glycosides Capsules.