Application of time-resolved fluoroimmunoassay for the detection of HBV serological markers
10.3969/j.issn.1673-4130.2014.21.033
- VernacularTitle:时间分辨荧光免疫技术在检测HBV血清学标志物中的应用
- Author:
Liying ZHANG
;
Pu CHEN
;
Yusheng PENG
;
Peng WANG
;
- Publication Type:Journal Article
- Keywords:
time-resolved fluorescence immunoassay;
enzyme-linked immunosorbent assay;
hepatitis B surface antigen;
serological markers
- From:
International Journal of Laboratory Medicine
2014;(21):2939-2940
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the value of time-resolved fluorescence immunoassay (TRFIA) and ELISA for the detection of HBV serological markers .Methods To detect the HBV serological markers in 359 samples with low concentrations of HBsAg by TRFIA and ELISA respectively ,and to compare the results of the two methods .Results The levels of HBV serological markers detected by TRFIA were higher than those detected by ELISA .The positive rates of HBsAg ,HBeAg and HBcAb detected by TR-FIA and ELISA were significantly different(P<0 .05) ,while those of HBsAb and HBeAb were not(P>0 .05) .Conclusion Com-pared with ELISA ,TRFIA has higher sensitivity in the detection of low HBsAg concentration samples ,and it is valuable to be ap-plicated in clinical .
