Molecular characterization of St ap hy lococcus aureus in Yanbian
- VernacularTitle:我国延边地区金黄色葡萄球菌分子特征研究
- Author:
Chunmei JIN
;
Hongbin CHEN
;
Meihua PIAO
;
Shunji XU
;
Wenqiang HE
;
Feifei ZHANG
;
Hui WANG
- Publication Type:Journal Article
- Keywords:
Staphylococcus aureus;
staphylococcal cassette chromosome mec;
Staphylococcus protein A typing;
multilocus sequence typing
- From:
Chinese Journal of Infection and Chemotherapy
2014;(5):425-428
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular epidemiology of Staphylococcus aureus in Yanbian area .Methods From March 2011 to June 2012 ,a total of 101 consecutive and non-duplicate strains of Staphylococcus aureus were collected from Yanbian Hospital .Genotypes of SCCmec ,spa,and multilocus sequence typing (MLST) were determined by PCR combined with DNA sequencing analysis .The pvl gene was detected by PCR .Results The most prevalent SCCmec type was type II (65 .0% ,39/60) ,followed by SCCmec type III (26 .7% ,16/60) .A total of 11 Spa types were identified for the MRSA strains ,including t2460 (55 .0% ,33/60) ,t030 (18 .3% ,11/60) ,t002 ,t324 ,and t632 (5 .0% ,3/60 each) .A total of 29 Spa types were identified for MSSA strains ,including t796 (14 .6% ,6/41) ,t309 (9 .8% ,4/41) ,and t126 (7 .3% ,3/41) . The pvl gene was identified in 5 stains .MRSA strains were classified into three types based on multilocus sequence typing (MLST) ,namely ST5 ,ST239 and ST72 .MLST-based MSSA types were more diverse ,including ST5 ,ST 25 ,ST 15 ,ST 59 ,ST 1 ,ST 7 ,ST 45 ,ST 22 ,and ST 188 .Conclusions ST5-MRSA-SCCmecII-t2460 and ST239-MRSA-SCCmecIII-t030 are the most prevalent MRSA clones in Yanbian area .Multiple prevalent MSSA clones are identified.
