Effect of etomidate preconditioning on etomidate-induced toxicity to rat adrenal cortical cells in vitro
10.3760/cma.j.issn.0254-1416.2014.06.009
- VernacularTitle:依托咪酯预处理对依托咪酯诱导大鼠肾上腺皮质细胞毒性的影响:离体实验
- Author:
Na'na Han
;
Shan Liu
;
Junyu Xiong
- Publication Type:Journal Article
- Keywords:
Etomidate;
Ischemic preconditioning;
Drug toxicity;
Adrenal cortex
- From:
Chinese Journal of Anesthesiology
2014;34(6):677-678
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of etomidate preconditioning on etomidate-induced toxicity to rat adrenal cortical cells in vitro.Methods After being primarily cultured for 7-9 days,the rat adrenal cortical cells at the exponential growth phase were seeded into 96-well culture plates (1 × 106 cells/ml) and cultured for 24 h.The cells were then randomly divided into 3 groups with 6 wells in each group:control group (group C),etomidate group (group E),and etomidate preconditioning group (group EP).In group E,the cells were incubated with 700 μmol/L etomidate for 24 h.In group EP,the cells were incubated with 1.25 μmol/L etomidate for 1 h,then washed out and incubated with 700 μmol/L eomidate for 24 h.The cell viability was determined by CCK-8 assay and the concentration of cortisol was determined by ELISA.Results Compared with group C,the cell viability and cortisol concentration were significantly decreased in E and EP groups.Compared with group E,the cell viability and cortisol concentration were significantly increased in group EP.Conclusion Etomidate preconditioning can reduce etomidate-induced toxicity to rat adrenal cortical cells in vitro.
