Bioinformatic analysis of the hsa-miR-1908 upstream promoter region

VernacularTitle:hsa-miR-1908上游启动子区的生物信息学分析
Author: Huining KUANGQIAN ; Jingyun LI ; Chenbo JI ; Xirong GUO ; Yuhui NI ; Meiyu XU
Publication Type:Journal Article
Keywords: miR-1908 promoter region; bioinformatic analysis; transcription factor binding site
From: Journal of Clinical Pediatrics 2014;(4):379-383
CountryChina
Language:Chinese
Abstract: Objective To predict the functions of hsa-miR-1908 promoter using various bioinformatic tools, and to provide clues for further study on transcriptional regulation mechanism of miR-1908 in human adipocytes. Methods The promoter se-quence of miR-1908 was obtained from Ensemble, and then the CpG islands and transcription factor binding sites were pre-dicted by a variety of online bioinformatic tools. Results The length of the miR-1908 promoter sequence was 1 458 bp. The CpG islands, which inhibited the transcription of miR-1908, were located at (438-756) bp, (836-937) bp and (979-1374) bp. Meanwhile, 15 transcription factor binding sites were found in the promoter sequence of miR-1908. Conclusions miRNA up-stream promoter related bioinformatics can not only improve the efficiency of microRNA promoter research, but also provide further important information on transcriptional regulation of miR-1908.