The ability to identify the yeast-like fungi by YST identification cards and pyrosequencing analysis
10.3760/cma.j.issn.1009-9158.2014.04.012
- VernacularTitle:YST鉴定卡与焦磷酸测序鉴定酵母样真菌的应用评价
- Author:
Yanwen GONG
;
Jixia LI
;
Chengjin HU
;
Yu'e ZHANG
;
- Publication Type:Journal Article
- Keywords:
Yeasts;
DNA,ribosomal spacer;
Sequence analysis,DNA
- From:
Chinese Journal of Laboratory Medicine
2014;37(4):290-293
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the ability of Vitek 2 Compact YST identification cards and pyrosequencing analysis for identifying the clinical isolates of yeast-like fungi.Methods Vitek 2 Compact YST identification cams and ITS1 region pyrosequencing analysis were used to identify the clinical isolates of yeast-like fungi at Jinan Military General Hospital in 2011.The strains which could not be identified to species by pyrosequencing analysis were identified again by the method of ITS1 region Sanger sequencing.The strains with inconsistent identified results by YST and pyrosequencing were identified again using API 20C.Results A total of 282 srtains were isolated from various clinical specimens for culturing in 2011.In addition to the three which could only be identified to similar species,other strains could be clearly identified to species by the method of ITS1 reverse pyrosequencing.Three strains which failed identified to species by pyrosequencing couldn't be distinguished fully by the method of Sanger sequencing.There were 7 strains unidentified,14 strains low discrimination(％ id ＜ 80％),and 6 strains misidentification using YST cams.The identification coincidence rate was 90.4％.The identification coincidence rates were various in different species of yeast-like fungi using YST cards.Conclusions The vast majority of clinical isolates of yeast-like fungi could be identified by ITS1 reverse pyrosequencing analysis.Vitek 2 Compact YST caRD basically meets the needs.But attention should be paid to the standard operating and regular internal quality control.For some rare species it is also needed to combine the source of specimen,colonies and characteristics to determine the identification results for the rare stains.
