Comparative evaluation of multilocus variable-number tandem-repeat analysis and pulsed-field gel electrophoresis for genotyping Salmonella enterica serovar Typhi isolates
10.3760/cma.j.issn.0254-5101.2014.04.003
- VernacularTitle:多位点串联重复序列分析和脉冲场凝胶电泳对伤寒沙门菌基因分型
- Author:
Weiyuan WU
;
Hui WANG
;
Jian LU
;
Yingxia LIU
;
Yuemei LU
;
Jinsong WU
;
Wenqing LI
;
Jin′e CHENG
;
Wenyuan WU
- Publication Type:Journal Article
- Keywords:
Salmonella enterica serovar Typhi;
Genotyping;
Multilocus variable-number tandem-repeat analysis;
Pulsed-field gel electrophoresis
- From:
Chinese Journal of Microbiology and Immunology
2014;(4):264-268
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the capability of multilocus variable-number tandem-repeat ( VNTR) analysis ( MLVA) and pulsed-field gel electrophoresis ( PFGE) for genotyping Salmonella enterica serovar Typhi (S.Typhi) isolates.Methods Five polymorphic VNTRs (SAL02,SAL11,SAL16,SAL20, and TR4699 ) that were observed in S.Typhi strains from previous studies were selected to establish MLVA . Twenty-one epidemiologically unrelated S.Typhi strains that were isolated from Shenzhen ,China from 2002 to 2007 were genotyped by the established MLVA , and the results were compared with those by PFGE . Results The Simpson′s index of diversity ( D value ) for all five different VNTRs ranged from 0.838 to 0.943 .A total of 19 MLVA profiles and 19 PFGE profiles were found , respectively .The D value for both MLVA and PFGE were 0.99 and the test results from two analyses were identical .Conclusion The five polymorphic VNTRs analysis could be used as an alternative typing scheme for epidemiologic investigation of S.Typhi infection .
