Efect of AcSDKP on collagen content and expression of NF-κB p65 in lung of rats with silicosis fibrosis
10.3969/j.issn.1671-8348.2013.26.024
- VernacularTitle:AcSDKP对硅肺纤维化大鼠胶原含量及NF-κBp65表达的影响
- Author:
Jingbo YAN
- Publication Type:Journal Article
- Keywords:
silicosis;
N-acetyl-seryl-aspartyl-lysyl-proline;
NF-κB p65
- From:
Chongqing Medicine
2013;(26):3139-3141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of N-acetyl-seryl-aspartyl-lysyl-proline (AcSDKP) on the synthesis of collagen and the expression of NF-κB p65 in lung of rats with silicosis fibrosis .Methods Rats were divided into 3 groups randomly :the control group(each rat was intratracheally instilled with 1 mL normal saline and killed after 8 weeks ,the silicotic model group(each rat was intratracheally instilled with 1 mL silica suspension and killed after 8 weeks ,the AcSDKP treated group(each rat was intra-tracheally instilled with 1 mL silica suspension and AcSDKP (800 μg · kg -1 · d-1 ) was administered into every rat ,then rats were killed after 8 weeks .Lung fibrosis in morphology and collagen content was observed by HE and vg staining and hydroxyproline as-say .The expression of NF-κB p65 was detected with the immunohistochemistry and Western blot .Results Compared with the con-trol group ,the quantity of silicon nodules ,collagen content and the expression of NF-κB p65were increased in the silicotic model group .Compared with the silicotic model group ,the quantity of silicon nodules ,collagen content and the expression of NF-κB p65 were decreased in the AcSDKP-treated group .Conclusion AcSDKP could inhibit the activity and expression of NF-κB p65 by strengthening the stability of I-κB ,which may influence the progress of alveolar inflammation and silicosis fibrosis .
