CD45 MOLECULE AND GENERATION OF EPIDERMAL γδ T CELLS FROM MICE
10.3969/j.issn.1000-8861.1999.02.002
- Author:
Qinchao CAI
;
Binghe WANG
;
- Publication Type:Journal Article
- Keywords:
CD45 protein tyrosine phosphatase (CD45 PTPase);
Dendritic epidermal T cells (DETC);
γδ T cell;
T cell generation
- From:
Immunological Journal
1999;(2):79-82
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the role of CD45 protein tyrosine phosphatase in γδ T cell development,we exa-mined whether Vγ3 dendritic epidermal T cells (DETC),a subset of γδ T cells uniquely reside in the murine epidermis were altered in the CD45-gene -deficient mice.In situ immunolabelling on epidermal sheets demonstrsted that the CD45-deficient mice had a normal density and immunophenotype of Vγ3 DETC in comparison to the wild-type control mice.RT-PCR revealed that similar levels of Vγ3 TCR mRNA were present in the epidermis of both CD45-deficient mice and wild-type controls.FCM showed no significant difference in the proportion of Vγ3 T cells in the epidermal cells between the two genotypes.In addition, the frequency of Vγ2 T cells, another subset of γδT cells in lymph nodes was normal in CD45-deficient mice.These results indicate that althouh CD45 is crucial for the development of αβΤ cells,it might be not necessary for the thymic maturation of γδ T cells including Vγ3 DETC and Vγ2 T cells.
