Mutation analysis of DC-SIGN promoter in chronic hepatitis B patients
10.3969/j.issn.1672-7347.2011.11.004
- VernacularTitle:慢性乙型肝炎患者DC-SIGN启动区变异分析
- Author:
Li CHEN
;
Congzhi LI
;
Xiujuan MENG
;
Ping'an ZHU
;
Deming TAN
- Publication Type:Journal Article
- Keywords:
DC-SIGN;
hepatitis B virus;
hepatitis B,chronic;
promoter region;
mutation
- From:
Journal of Central South University(Medical Sciences)
2011;36(11):1052-1058
- CountryChina
- Language:Chinese
-
Abstract:
To investigate whether there is mutation in DC-SIGN promoter region in patients with chronic hepatitis B (CHB) and healthy persons previously infected with hepatitis B virus (HBV) and to explore the relationship between the mutation in dendritic cell-specific intercellular adhension molecule-3-grabbing nonintegrin (DC-SIGN) promoter region and HBV.Methods The studied population was composed of two cohorts:47 CHB patients and 20 healthy persons previously infected with HBV.The mutation in DC-SIGN promoter region was detected with PCR,single-stranded conformational polymorphism and heteroduplex analysis,cloning,sequencing and aligning the published DC-SIGN promoter sequence.Results The characteristic mutation within DCSIGN promoter region in HBV infected individuals was observed.In the DC-SIGN promoter region,4 hot spot mutations located in positions - 139,- 142,- 222,and - 336 were observed in the CHB patients,but only 1 spot mutation located in position - 139 was observed in the healthy persons previously infected with HBV.The -336C which was absent in the healthy persons previously infected with HBV was shown in 11 CHB patients (23.40％).The - 139T was far more frequent in the healthy persons previously infected with HBV ( 100％ ) than in the CHB patients (34.04％).Conclusion In the DC-SIGN promoter region,-336C may be a genetic risk factor for developing CHB,but -139T may be associated with protection against HBV.
