The effect of injection of bone mesenchymal stem cell into the joints of collagen-induced arthritis rats
10.3760/cma.j.issn.1007-7480.2011.11.003
- VernacularTitle:骨髓间充质干细胞关节腔注射治疗胶原诱导性关节炎的实验研究
- Author:
Xi-lan YANG
;
Peng ZHANG
;
Mei-jiang FENG
;
Hui-wei HE
;
Chen QU
;
Jing-jing HUANG
;
Xiang LU
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Injection,intra-articular;
Arthritis,experimental
- From:
Chinese Journal of Rheumatology
2011;15(11):736-738
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the effect of Bone mesenchymal stem cells(BMSCs) intraarticular injection on the pathological development of collagen-induced arthritis(CIA) rats.MethodsCIA Wistar rats were divided into 2 groups:the saline injection control group and the BMSCs injection group.BMSCs were isolated from normal rats and cultured in vitro till P2 generation,which were injected into the affected the ankle joint consequently.After 2,4,8,12 weeks of processing,the following indexes were observed:arthritis score and X-ray.Twelve weeks later,all of the rats were sacrificed and the following histological indexes were evaluated:cartilage destruction and cartilage extracellular matrix secretion.Paired samples t-test was used for the statistical comparison.ResultsAll the arthritis indexes of the BMSCs group were much lower.than those of the saline control group.The arthritis scores were 3.18±0.62 vs 3.84±0.35 (at week 2),3.45±0.28 vs 5.96±0.48(at week 4),3.86±0.23 vs 6.75±0.36(at week 8),4.23±0.43 vs 7.86±0.66 (at week 12) respectively.X-ray scores were 2.04±0.21 vs 2.72±0.15(at week 2),2.52±0.47 vs 4.06±0.38 (atweek 4),3.56±0.29 vs 4.35±0.36 (at week 8),3.73±0.43 vs 4.86±0.62(at weekl2) respectively and the histological evaluation indexes were 2.34±0.22 vs 3.52±0.55 (at week 12).ConclusionBMSCs injection may inhibit the pathological development of CIA rats,and it may be a promising approach for the treatment of rheumatoid arthritis.
