Effect of NGF on the sperm motility of human in vitro
10.3969/j.issn.1673-4130.2012.20.001
- VernacularTitle:NGF体外对精子活力的影响
- Author:
Kai LIN
;
Cuige SHI
;
Yongqi ZHAO
;
Shuhong LIU
;
Ming FAN
- Publication Type:Journal Article
- Keywords:
infertility,male;
nerve growth factor;
sperm motility
- From:
International Journal of Laboratory Medicine
2012;33(20):2433-2437
- CountryChina
- Language:Chinese
-
Abstract:
Objective Motility is an important physiological characteristic of a mature sperm.Nerve growth factor(NGF) is a protein essential for the development,maintenance and survival of the peripheral and central nervous systems.NGF and its receptors TrkA and p75 are widely expressed in the testis,accessory reproductive organ,and the epididymal sperms.In the present study,we investigated the role of NGF on human sperm motility.Methods Use 0.1,1 and 10 μmol/L concentrations of NGF,on sperm motility study to investigate the optimal concentration.Use CASA to detect Sperm motility changes every 10 minutes in an hour after 10 μmol/L NGF was added to the semen.Results The parameters of sperm motility increased after NGF incubation had significant difference, in particular,VAP,VSL,VCL,BCF and LIN mean were significantly increased more than 32%.MAD,STR,ALH and WOB mean had no notable difference.Furthermore,NGF promotes the sperm motility in a time- and dose- dependent manner.In addition,the enhancement of NGF on sperm motility was more stronger than those of sperm culture medium.Conclusion Our findings suggest that NGF plays a promoted role in human sperm motility.
