Effect of Chaihu Shugan San and its components on expression of ERK1/2 mRNA in the hippocampus of rats with chronic mild unpredicted stress depression
10.3969/j.issn.1672-7347.2011.02.001
- VernacularTitle:柴胡疏肝散及其拆方对慢性应激抑郁大鼠模型海马组织ERK1/2 mRNA表达的影响
- Author:
Su'e WANG
;
Suiyu HU
;
Chunhu ZHANG
;
Juan QIU
;
Yunhui LI
;
- Publication Type:Journal Article
- Keywords:
Chaihu Shugan San;
depression;
ERK1/2;
real-time fluorescent quantitative PCR;
regularity of recipe composition
- From:
Journal of Central South University(Medical Sciences)
2011;36(2):93-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the antidepressant effect and mechanism of Chaihu Shugan San (CHSGS) composition, a compound traditional Chinese herb medicine and its components. Methods Rats were randomly divided into 6 groups: normal control group, model control group, a CHSGS group, a component I group, a component II group and a fluoxetine control group. The depression model was replicated by chronic unpredictable mild stress and single house for 28 d. Behavioral scores of the rats were detected by Open-field test and sucrose solution consumption test, and ERK1/2 mRNA expression in the hippocampus tissue was assayed by real-time fluorescent quantitative PCR. Results ERK1/2 mRNA expression was down-regulated in the depression model group compared with the normal control group (P<0.01). Compared with the model group, ERK1/2 mRNA expression in the CHSGS group and fluoxetine group was both up-regulated (P<0.05 or P<0.01); and only ERK1 mRNA expression in the component I group was up-regulated (P<0.05). No significant difference existed between the component II group and the model group (P>0.05). Conclusion Isolated-living condition and chronic mild unpredictable stress can down-regulate the expression of ERK1/2 mRNA in the hippocampus tissue. CHSGS may exert an antidepressant effect through increasing the expression of ERK1/2 mRNA in the hippocampus, component I may play an important role in its antidepression effect, while compatibility of the use of component II can enhance the antidepressant efficacy.
