Influence of intraspinal implantation of pSVPoMcat genetically modified schwann cell on regeneration of injured spinal cord
- VernacularTitle:pSVPoMcat微基因修饰雪旺细胞脊髓内移植对操作脊髓再生的影响
- Author:
Ligang CHEN
;
Lida GAO
;
Boyong MAO
;
Min LU
;
Xun LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2001;5(16):152-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to observe the role of genetically modified Schwann cell (SC) with pSVPoMcat in the regeneration of injured spinal cord.Method The cells were implanted into the spinal cord.Ninety SD rats were used to establish a model of hemi- transection of spinal cord at the level of T8,and were divided into three groups,randomly, that is,pSVPoMcat modified SC implantation(Group A), SC implantation(Group B),and without cell implantation as control(Group C).After three months the presence of axonal regeneration of the injured spinal cord was examined by means of horseradish peroxidase(HRP)retrograde labeling technique and stereography.Result The results indicated that HRP labeled cells in Group A and B could be found in the superior region of injured spinal cord and the brain stem such as the red nuclei and oculomotor nuclei. The density of ventral horn neurons of the spinal cord and the number of myelinated axons in 100 μ m of the white matter was A >B >C group.Conclusion In brief,the pSVPoMcat modified SC intraspinal implantation could promote regeneration of the injured spinal cord.
