Antisense oligonucleotide mediated inhibition on telomerase activity in gallbladder carcinoma cell
- VernacularTitle:反义寡核苷酸对胆囊癌细胞端粒酶活性作用的实验研究
- Author:
Jin Bin
;
JINAG Xi-hong
;
WANG Wei
;
XU Ke-sen
;
SHI Zhao-hui
- Publication Type:Journal Article
- Keywords:
Gallbladder neoplasms;
Telomerase;
Oligoribonucleotides,antisense
- From:
Chinese Journal of Current Advances in General Surgery
2005;8(1):27-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the antisense oligonucleotide mediated inhibition on telomerase activity and cell proliferation of GBC-SD cell.Methods:We design the antisense,sense,and random oligonucleotide with phosphoric acid modification for the hTR(Human Telomerase RNA)template sequence.MTT and PCR methods were used to observe the inhibition on telomerase activity and cell proliferation of GBC-SD cell ,and fibroblast cells were used as control group.Results:PS-ODN can lead to the reduction of cell survival rate of GBC-SD cell,wich dosage dependence.Tne experimental group cell detected by scanning electron appeared apoptotic feature.Conclusion:PS-ODN can inhibit telomerase activity of GBC-SD cell effectively and induce the cell apoptosis.
