OPTIMISATION AND VALIDATION OF AN IN VITRO EXTRACELLULAR ELECTROSTIMULATING DEVICE
- VernacularTitle:离体的细胞外电刺激装置的优化和验证
- Author:
Rong XIA
;
Francois Berger
;
Alim-Louis Benabid
- Publication Type:Journal Article
- Keywords:
Parkinson's disease;
high frequency electrical stimulation;
cell culture;
metabolism
- From:
Chinese Journal of Neuroanatomy
2007;23(5):489-494
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the mechanisms of high frequency stimulation ( HFS), we designed an in vitro device to deliver extracellular electrical stimulation to live cells in culture, using the same parameters as in the surgical treatment of Parkinson's disease (PD). The system was made of a 24 wells culture plate electrified with titanium wire bridges or two titanium wires placed parallel in a culture dish of 10 centimetres diameter. The extremities of the series of wells were connected to a constant current generator. The result showed that this system had no toxicity on GH3 and PC12 cell cultures. Preliminary experiences demonstrate that this system is suitable for the study with DNA and protein chip technologies.
