The Age-related Changes of the Expression of Caspase-3 and the apoptosis States of Neurons in Primary Auditory Cortex(AI) of C57BL/6J Mice
- VernacularTitle:C57BL/6J小鼠初级听皮层神经元凋亡与caspase-3表达的年龄相关性改变
- Author:
Hongbo LI
;
Jichuan CHEN
;
Changyou JI
;
- Publication Type:Journal Article
- Keywords:
Caspase-3;
Primary auditory cortex;
Apoptosis;
Hearing loss;
Age- related
- From:
Journal of Audiology and Speech Pathology
2009;17(3):248-251
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study is to study the age related changes of the expression of caspase-3 and the apoptosis states of neurons in primary auditory cortex of 15 young C57BL/6J mice(2 months, 15～20 g) and 15 old C57BL/6J mice(10 months, 50～60 g) and to determine probable physical effects underlying these changes. This paper also discusses the relationship of caspase-3 and apotosis states in primary auditory cortex, the possible role of caspase-3 in primary auditory cortex and the pathogenesis of presbycusis. Methods The immunohistochemical methods were applied to explore the differences of the expression of caspase-3 and the apoptosis states determined by TUNEI. method in the primary auditory cortex between young and old C57BL/6J mice. Results The expression of caspase-3 and apoptosis in Al of old C57BL/6J mice was significantly higher than that in the counterpart of young C57BL/6J mice. Conclusion The results presented a direct morphological evidence for the strengthening of caspase-3 in the primary auditory cortex in old C57BL/6J mice. This method has an important role in the formation and development of presbycusis. The expression of caspase-3 participated in the regulatory procedure of apoptosis and may be a possible factor of the etiologies of presbycusis.
