Screening for methylation-silenced genes in acute myeloid leukemia HL-60 cell line by a quantitative proteomic approach
10.3969/j.issn.1672-7347.2010.07.001
- VernacularTitle:采用定量蛋白质组学技术筛选急性髓系白血病HL-60细胞的甲基化相关基因
- Author:
Can'e TANG
;
Tan TAN
;
Yanhua XIAO
;
Lin RUAN
;
Cui LI
;
Fang PENG
;
Maoyu LI
;
Pengfei ZHANG
;
Hong YI
;
Zhiqiang XIAO
- Publication Type:Journal Article
- Keywords:
HL-60;
methylation;
two-dimensional fluorescence difference gel electrophoresis;
5-aza-2-deoxycytidine
- From:
Journal of Central South University(Medical Sciences)
2010;35(7):641-648
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen for new methylation association genes in HL-60 to reveal the pathogenesis of leukemia, and provide important theoretical and scientific basis for the prevention and cure of leukemia. Methods Two-dimensional fluorescence difference gel electrophoresis (F-2D-DIGE) was performed to separate the total proteins from acute myelogenous leukemia (AML) cell line HL-60 cells with or without 5-aza-2-deoxycytidine (5-aza-2-dC) treatment. Imaging software Decyder 6.5 and PDQuest were used to detect the differential expression protein spots, and matrix-assisted laser desorption/ionizaion time-of-flight mas spectrometer (MALDI-TOF MS) was adopted to identify the differential expression proteins. Results F-2D-DIGE maps of 5-aza-2-dC-untreated HL-60 and-treated HL-60 cells were established. A total of 53 differential protein spots were detected, and 35 differential proteins were successfully identified. Of the identified proteins, 32 proteins were up-regulated, and 3 proteins were down-regulated in HL-60 cells after 5-aza-2-dC treatment.Conclusion Thirty-five differential proteins may be associated with methylation in HL-60 cell line, which provides the important clues for epigenetic study of leukemia.
