The effect of cyclindependent kinase 2 On herpes simplex virus replication
- VernacularTitle:细胞周期蛋白依赖性激酶2活性与单纯疱疹病毒复制关系的研究
- Author:
Ze-hong
;
GUAN SHORGEN
- Publication Type:Journal Article
- Keywords:
Herpes simplex virus;
Cyclindependent kinase 2;
Virus replication
- From:
Chinese Journal of Microbiology and Immunology
2008;28(8):703-706
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of cyclindependent kinase(CDK)2 on the replication of herpes simplex virus(HSV).Methods Dominant-negative CDK2 expressing cell line HT29Tet-On dn-CDl(2 was used as host cells to determine the role of CDK2 in HSV rephcation.HT29Tet-0n dn-CDK2 cells were infected with HSV.The activity of CDK2 Was determined by the immunoprecipitate and autoradiograph before and after HSV infection.The growth curve of HSV Was conducted.Results The CDK2 activity was induced after infection for 6 h and reached the hishest level at 9 h.HSV replicated rapidly and productively after the CDK2 was induced.Coatiusion CDK2 is required for the replication of HSV,and HSV replicates in host cells by inducing the CDK2 activity.
