Expression of heme oxygenase-1 in peritoneal metastasis of gastric cancer and its significance HUI
10.3760/cma:j.issn.0254-1432.2009.09.003
- VernacularTitle:血红素氧合酶-1在胃癌腹膜转移中的表达及意义
- Author:
Liang-liang HUI
;
Fei-hu BAI
;
Li YANG
;
Kai-chun WU
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Neoplasms metastasis;
Hemeoxygenase-1;
Drug resistance,multiple
- From:
Chinese Journal of Digestion
2009;29(9):521-524
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significance of heme oxygenase-1(HO-1) in gastric adenocarcinoma and its peritoneal metastatic tissues, as well as drug-resistant cell strains. Methods The expression of HO-1 in patients with (n=68) or without (n=46) peritoneal metastasis of gastric adenocarinoma was examined. The expression of HO-1 in cancerous tissue, peritoneal metastatic foci, and normal peritoneum was detected by immunohistochemistry. The expression of HO-1 protein in metastatic foci and drug-resistant cell strains was measured by Western blotting. Results The positive expression of HO-1 was 39.7% (27/68) in gastric adenocarcinoma tissues with metastasis and 41.2% (28/68) in peritoneal metastatic tissues, which was significantly higher than that in normal peritoneum (0%,0/68,P<0.01) and gastric adenocarcinoma tissues without metastasis (21.7%, 10/46, P<0.05). The Western blot study showed that the HO-1 expression in metastatic tissues was higher than that in normal peritoneum (P<0.05). The expression of HO-1 protein was markedly increased in GC9811-P drug-resistant cell strains compared with its parental cell strains (P<0.05). Conclusions The increased expression of HO-1 may be involved in the peritoneal metastasis of gastric adenocarcinoma, and related to the malignant potential. The underlying signal pathways in neopastic epithelium may also be related to the multi-drug resistance.
