Production and Characterization of Monoclonal Antibodies to Bluetongue Virus

Author: Veerakyathappa Bhanuprakash ; Madhusudhan Hosamani ; Vinayagamurthy Balamurugan ; Pradeep Narayan Gandhale ; Gnanavel Venkatesan ; Raj Kumar Singh
Publication Type:Journal Article
Keywords: Bluetongue virus; Competitive ELISA; Enzyme-linked immunosorbent assay; Monoclonal antibody; India
From: Virologica Sinica 2011;26(1):8-18
CountryChina
Language:Chinese
Abstract: In the present study, a total of 24 Mabs were produced against bluetongue virus (BTV) by polyethyleneglycol (PEG) mediated fusion method using sensitized lymphocytes and myeloma cells. All these clones were characterized for their reactivity to whole virus and recombinant BTV-VP7 protein, titres, isotypes and their reactivity with 24 BTV-serotype specific sera in cELISA. Out of 24 clones, a majority of them (n = 18)belong to various IgG subclasses and the remaining (n = 6) to the IgM class. A panel of eight clones reactive to both whole BTV and purified rVP7 protein were identified based on their reactivity in iELISA. For competitive ELISA, the clone designated as 4A10 showed better inhibition to hyperimmune serum of BTV serotype 23. However, this clone showed a variable percent of inhibition ranging from 16.6% with BTV 12 serotype to 78.9% with BTV16 serotype using 24 serotype specific sera of BTV originating from guinea pig at their lowest dilutions. From the available panel of clones, only 4A 10 was found to have a possible diagnostic application.