Effects of Tannins Extracted from Phyllanthi Fructus on the Activity of Cytochrome P450 Enzymes in Rats

VernacularTitle:藏药余甘子鞣质部位对大鼠肝微粒体代谢酶P450活性的影响
Author: Yaping CUI ; Guanghui YANG ; Qi QI ; Lingfang WU ; Wenjing CHEN ; Wenyi LIANG ; Shi LI ; Lanzhen ZHANG
Keywords: Tannins extracted from Phyllanthi Fructus; rat liver microsomes; metabolic enzyme P450; activity
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2016;18(7):1159-1165
CountryChina
Language:Chinese
Abstract: This study aimed to investigate the effects of tannins extracted from Tibetan medicine Phyllanthi Fructus on cytochrome P450 enzyme of liver microsomes in rats.Cocktail probe substrates were incubated with liver microsomes in vitro.Metabolic elimination percentages of the six probe substrates,including dapsone,dextromethorphan hydrobromide,coumarin,phenacetin,chlorzoxazone and tolbutamide,were determined by HPLC.The effects of tannins extracted from Tibetan medicine Phyllanthi Fructus on the enzymatic activity of rat liver microsomal P450s was evaluated.It was found that tannins extracted from Phyllanthi Fructus did not impact P450 enzymes.The IC50 values of CYP3A4 and CYP1A2 were over 200 μg·mL-1,while the IC50 value of CYP2C9 was superior to 500 tg·mL-1.In conclusion,Tannins extracted from Tibetan medicine Phyllanthi Fructus did not significantly affect cytochrome P450 enzymes.