Effects of Eerdun-Wurile on the Expressions of BDNF and NGF in the Prefrontal Cortex in Middle Cerebral Artery Occlusion / Reperfusion (MCAO / R) Injured Rats
10.11842/wst.2016.07.022
- VernacularTitle:额尔敦-乌日勒对MCAO/R大鼠脑前额叶皮质BDNF及NGF表达的影响
- Author:
Hua LIAN
;
Chunjie MA
;
Relebagen HU
;
Chao LI
;
Haiguang TIAN
;
Zhibin XIAO
- Keywords:
Eerdun-Wurile;
middle cerebral artery occlusion / reperfusion model;
brain-derived neurotrophic factor;
nerve growth factor
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2016;18(7):1212-1218
- CountryChina
- Language:Chinese
-
Abstract:
The present study aimed to explore the effects of Eerdun-Wurile on brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) expressions in the prefrontal cortex of MCAO / R injury rats.Sixty SD male rats of SPF grade were selected to establish the model of MCAO / R with Zea-Longa thread occlusion,and divided into five groups at random:the sham operation group,the model group,the nimodipine group and the Eerdun-Wurile group.After modeling,rats were anesthetized for preparing the brains.The pathomorphological changes of the brains were evaluated by immunohistochemical techniques,such as HE staining and SP.The protein and mRNA expressions of BDNF and NGF in the prefrontal cortex of rats were detected by ELISA and RT-PCR,respectively.As a result,compared with the model group,it was found that the number of necrotic cells in the prefrontal cortex were significantly reduced in the Eerdun-Wurile group (P ＜ 0.05),while the mRNA and protein expressions of BDNF and NGF were significantly increased (P ＜ 0.05).In conclusion,the BDNF and NGF expressions in the prefrontal cortex were up-regulated for stimulating the activation of astrocytes and protecting the neurons with the treatment of Eerdun-Wurile in MCAO / R injured rats,which may be the mechanism of the treatment of Eerdun-Wurile for white vein disease.
