Determination of Cucurbitacin IIa in Rat Plasma by HPLC and its Pharmacokinetic Study
- VernacularTitle:HPLC测定大鼠血浆中雪胆甲素的浓度及其药动学研究
- Author:
Chao SHI
;
Qingyu CAI
;
Shu LI
- Publication Type:Journal Article
- Keywords:
Cucurbitacin IIa;
HPLC;
Pharmacokinetics
- From:
China Pharmacist
2015;(11):1873-1875,1878
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a sensitive and fast HPLC method for determining cucurbitacin IIa in rat plasma and investigate the pharmacokinetic characteristics after intravenous administration. Methods: The analysis was performed on a Dikma Diamonsil C18 column (250 mm × 4. 6 mm, 5 μm) at 35℃ with the mobile phase of acetonitrile-water (45∶ 55, v/v) and the UV detection at 212 nm. The plasma samples were collected after intravenous administration at different time points and measured by HPLC. The pharmaco-kinetic parameters were calculated by DAS 2. 0 software. Results: The calibration plot of cucurbitacin IIa was linear over the range 0. 146-14. 060 μg · ml-1 . The range of method recovery was 99. 02%-104. 22% and that of extraction recovery was 84. 74%-86. 80%. The intra-and inter-day precision were both less than 5%. The stability met the requirements. The main pharmacokinetic pa-rameters were showed as follows:t1/2β(h):0.732 ±0.151, 0.681 ±0.055,0.667 ±0.064;Vd (L·kg-1):0.147 ±0.089, 0.131 ±0.095,0.153±0.047; Cl(L·h-1·kg-1): 0.287±0.031,0.304±0.063,0.318±0.029andAUC0→∞(mg·h·L-1):3. 646 ± 1. 124,4. 916 ± 1. 227,9. 385 ± 1. 419. Conclusion: The validated method is successfully applied in basic pharmacokinetic study in rat plasma after intravenous administration. The plasma concentration-time curves at three dosages are all fitted three-compart-ment model. Within the examined dose range, the pharmacokinetics of cucurbitacin IIa in rat is based on linear pharmacokinetics.
