Preparation and Drug Release in vitro of Danshensu Liposomes
- VernacularTitle:丹参素脂质体的制备及体外释放度研究
- Author:
Zhouxin JI
;
Linlin LIU
;
Yiyang LI
;
Xiaoyun DENG
;
Liao CUI
;
Yingnian LV
- Publication Type:Journal Article
- Keywords:
Danshensu;
Liposomes;
Entrapment efficiency;
Particle size;
Release in vitro
- From:
China Pharmacist
2015;18(10):1649-1651,1660
- CountryChina
- Language:Chinese
-
Abstract:
Objective:o prepare Danshensu liposomes and investigate drug release characteristics in vitro. Methods: Danshensu liposomes were prepared by a reverse-phase evaporation method. The encapsulation efficiency was used as the index, an orthogonal test was adopted to investigate the effect of concentration of soybean lecithin, ratio of lipid-Danshensu and pH value of solution on the preparation procedure of Danshensu liposomes. The particle size of the liposomes was also investigated by a transmission electron micro-scope ( TEM) . The concentration of Danshensu was determined by HPLC, and the difference of release characteristics in Danshensu li-posomes and Danshensu solution was measured by a dialysis method. Results:The optimum preparation technology was as follows:the concentration of soybean lecithin was 40 mg·ml-1 ,the ratio of drug-lipid was 1: 10,and the pH value of solution was 6. 6. The mor-phology of the prepared liposomes showed spheric structure with uniform diameter, and the average particle size was ( 174 ± 36 ) nm and the encapsulation efficiency was 38. 9%. The linear range of Danshensu was 2. 0-20. 0 mg·L-1(r=0. 9984). The drug release of liposomes in vitro was slower than that of free Danshensu solution in 24 h. Conclusion:Danshensu liposomes with fine morphology have sustained release property.
