Influence of Sanguisorba Officinalis Tannins Extract on Proliferation of Human Renal Tubular Epithelia Cells Induced by TGF-β1
- VernacularTitle:地榆鞣质提取物对 TGF-β1 诱导人肾小管上皮细胞增殖的影响
- Author:
Huilan YI
;
Benhong ZHOU
;
Jie TU
;
Yue WU
- Publication Type:Journal Article
- Keywords:
Sanguisorba officinalis tannins extract;
Transforming growth factor-β1;
Human renal epithelia;
Proliferation
- From:
China Pharmacist
2015;18(10):1668-1670,1673
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the preventive effect of Sanguisorba officinalis on renal fibrosis by observing the influence of San-guisorba officinalis tannins extract (STE) on the proliferation of human renal epithelia (HK-2) induced by transforming growth factor-β1 ( TGF-β1 ) . Methods:HK-2 cells were cultured in DMEM medium with high glucose containing 10% fetal bovine serum. The cul-tured cells were divided into 5 groups, including the blank control group, TGF-β1 group (5 ng· ml-1 TGF-β1), intervention group 1 (5 ng·ml-1 TGF-β1 +12. 5μg·ml-1 STE), intervention group 2 (5 ng·ml-1 TGF-β1 +25μg·ml-1 STE) and intervention group 3(5 ng·ml-1 TGF-β1 +50 μg·ml-1 STE). The changes of cell morphology were observed under an inverted microscope and the in-fluence of SET on the cell proliferation was detected by CCK8 assay. Results: TGF-β1 could significantly induce the proliferation of HK-2 and promote the cell fibrosis with significant difference when compared with the control group (P<0. 05). However, after trea-ted with STE, the cell proliferation was inhibited obviously (P<0. 05) and the cells morphology tended to be normal in a dose-depend-ent manner. Conclusion:STE can inhibit the proliferation of HK-2 and prevent renal fibrosis to some extent.
