Determination Optimization of Peimine and Peiminine in Three Varieties of Fritillaria
- VernacularTitle:3种贝母类药材贝母素甲和贝母素乙含量测定方法的优化
- Author:
Ling XU
;
Wenbing PENG
;
Songqiao FENG
- Publication Type:Journal Article
- Keywords:
Fritillaria;
Content determination;
Optimization;
Peimine;
Peiminine;
HPLC-ELSD
- From:
China Pharmacist
2015;(8):1299-1301,1302
- CountryChina
- Language:Chinese
-
Abstract:
To establish an optimized determination method for the alkaloids peimine and peiminine in three varieties of Fritillaria. Methods:The samples were refluxed in a mixed solution, the pH value of the mixed solution was adjusted, and then the solution was extracted by ether to obtain the test solution. A Shim-pack VP-ODS column (4. 6 mm × 250 mm,5 μm) was used, the mobile phase consisted of acetonitrile-0. 02% diethylamine(75∶25), and the flow rate was 1. 0 ml·min-1. An Alltech 2000ES ELSD detector was used, the drift tube temperature and the flow rate of nitrogen carrier gas in the ELSD was set at 93℃ and 2. 4 L·min-1 , respectively. The regression equation was calculated between the logarithm values of the reference solution concentration and the corre-sponding peak area. Results:The method could effectively extract the alkaloids from the samples. Peimine and peiminine were well separated by the method, and showed good linearity within the respectively given ranges. The average recovery of the alkaloids in the three varieties of Fritillaria was within the range of 95. 22%-103. 51%. Conclusion: The method is simple,convenient and universal with easy operation,which provides references for the determination of alkaloids in the other varieties of Fritillaria.
