Isolation and Preparation of Phloroglucinol Derivatives from Dryopteris Fragrans by Semi-preparation HPLC
- VernacularTitle:半制备高效液相色谱法分离制备香鳞毛蕨中间苯三酚类衍生物
- Author:
Guoqiang SONG
;
Guotong DENG
;
Wenzhao DU
;
Guanfeng DU
;
Jiangchen CHEN
;
Zhibin SHEN
- Publication Type:Journal Article
- Keywords:
Semi-preparation HPLC;
Dryopteris fragrans;
Phloroglucinol derivatives;
Aspidin BB;
Aspidin PB
- From:
China Pharmacist
2015;(4):545-547,548
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate and prepare the reference substances of phloroglucinol derivatives from Dryopteris fragrans by semi-preparation HPLC. Methods:After reflux extraction of Dryopteris fragrans with petroleum ether,the extracting solution was con-centrated and separated by silica gel column chromatography,and then isolated by semi-preparation HPLC. The isocratic elution was carried out using acetonitrile as the mobile phase at 3. 0 ml·min-1 and the injection volume was 0. 5 ml. Two phloroglucinol deriva-tives were isolated. Results:The chemical structure of the two phloroglucinol derivatives respectively was aspidin BB with the purity of 98. 81% and aspidin PB with the purity of 98. 57% by ultra high performance liquid chromatography. Conclusion:The isolation of as-pidin BB and aspidin PB by semi-preparation HPLC is simple and fast with the purity over 98%,which can be used to prepare the ref-erence substances.
