In vitro Anti-tumor Effect of Extracts from Different Parts of Tricholoma Matsutake Sing
- VernacularTitle:松茸不同提取部位的体外抗肿瘤作用
- Author:
Gang LIU
;
Cai SHI
;
Hui WANG
;
Xianxi GUO
;
Jie WU
;
Benhong ZHOU
- Publication Type:Journal Article
- Keywords:
Tricholoma matsutake Sing.;
Anti-tumor;
MTT
- From:
China Pharmacist
2015;(5):701-703,704
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the anti-tumor effect of different extracts of Tricholoma matsutake Sing. in vitro. Methods:MTT method was employed to study the inhibitory effect of ethanol extracts from different parts of Tricholoma matsutake Sing. and the polysaccharides extracts on HepG-2 cells, MCF-7 cells and Hela cells. FCM assay was used to detect HepG-2 and Hela cell apoptosis induced by the polysaccharides extracts. Results:The polysaccharides extracts of Tricholoma matsutake Sing. could inhibit the prolif-eration of the three tumor cells with IC50 of 53. 77 μg·mL-1(HepG-2), 40. 04 μg·mL-1(Hela) and 100. 65 μg·mL-1(MCF-7), respectively. The inhibitory effect of the polysaccharides extracts showed a time-and dose-dependent manner. The tumor cell apoptosis could be induced by the polysaccharides extracts. Conclusion:The maln anti-tumor effective extracts of Tricholoma matsutake Sing. in vitro is the polysaccharides extracts, which show significant anti-tumor activities in vitro and can induce tumor cell apoptosis.
