Determination of Ginsenoside Rg1, Ginsenoside Re and Ginsenoside Rb1 in Yi’ nianjin by UPLC
- VernacularTitle:UPLC法测定一捻金中人参皂苷 Rg1、人参皂苷 Re、人参皂苷Rb1的含量
- Author:
Xiao SUN
;
Guangsheng YANG
;
Xiaolong ZHANG
;
Xinfei MA
- Publication Type:Journal Article
- Keywords:
UPLC;
Yi’ nianjin;
Ginsenoside Rg1;
Ginsenoside Re;
Ginsenoside Rb1
- From:
China Pharmacist
2015;(1):159-160,161
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a UPLC method for the determination of ginsenoside Rg1 , ginsenoside Re and ginsenoside Rb1 in Yi’ nianjin. Methods: The column was ZORBAX Eclipse Plus C18 (2. 1 mm × 50 mm,1. 8 μm), the flow rate was 0. 21 ml· min-1 , the column temperature was 30℃, the mobile phase consisted of acetonitrile-water with gradient elution, the detection wave-length was 203nm, and the sample size was 1μl. Results:The linear range of ginsenoside Rg1 was 0. 020 3-0. 303 9 mg·ml-1 ( r=0. 999 6), and the average recovery was 99. 05% (RSD=1. 3%, n=6). The linear range of ginsenoside Re was 0. 020 2-0. 302 7 mg·ml-1(r=0. 999 8), and the average recovery was 101. 31% (RSD=1. 1%, n=6). The linear range of ginsenoside Rb1 was 0. 020 3-0. 305 1 mg·ml-1(r=0. 999 8), and the average recovery of 100. 71% (RSD=0. 9%, n=6). Conclusion: The method is simple and accurate, and can determine the three components simultaneously. The method can be used in the quality control of Yi’ nianjin.
