Determination of Notoginsenoside R1,Ginsenoside Rg1 and Ginsenoside Rb1 in Compound Huanggen Gran-ules by HPLC
- VernacularTitle:HPLC法测定复方黄根颗粒中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1含量
- Author:
Hui YANG
;
Guangyao LAO
;
Dezhi TANG
- Publication Type:Journal Article
- Keywords:
Compound Huanggen granules ( sugar free);
Notoginsenoside R1;
Ginsenoside Rg1;
Ginsenoside Rb1;
HPLC
- From:
China Pharmacist
2015;(1):55-57,58
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To set up a method for simultaneously determining the content of notoginsenoside R1 , ginsenoside Rg1 and ginsenoside Rb1 in compound Huanggen granules ( sugar free) . Methods:The HPLC method was carried out on an Agilent ZORBAX SB-C18(250 mm ×4.6 mm,5 μm)column. The mobile phase was acetonitrile-water with gradient elution. The flow rate was 1.0 ml· min-1 . The detection wavelength was at 203 nm. The column temperature was set at 25℃ and the sample size was 10 μl. Results:The linearity of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 was within the range of 1. 6-10. 0 μg·ml-1(r=0. 999 6), 6. 3-39. 3 μg·ml-1(r=0. 999 8) and 6. 3-39. 7 μg·ml-1(r=0. 999 7), respectively. The average recovery was 98. 81%(RSD=1. 20%),99. 93%(RSD=0. 93%) and 99. 22%(RSD=0. 87%)(n=6) , respectively. Conclusion: The method is simple, repro-ducible and specific, which can be used in the quality control of the preparation.
