Neuronal plasticity and the changes of microenvironment at the ischemic neocortical penumbra after cere- bral artery occlusion in rats
10.3936/j.issn.1002-0152.2015.01.004
- VernacularTitle:大脑中动脉栓塞大鼠缺血半暗区神经可塑性蛋白及神经因子的表达
- Author:
Tian TIAN
;
Niancong CHE
;
Jianfei LONG
;
Lei WANG
;
Haiyan ZOU
- Publication Type:Journal Article
- Keywords:
Middle cerebral artery;
Ischemic penumbra;
Nutrient factor;
Inhibitory factor
- From:
Chinese Journal of Nervous and Mental Diseases
2015;(1):15-19,25
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate the effects of nerve nutrient factors and inhibitory factors on neuronal regen? eration and axonal reconstruction at ischemic neocortical penumbra after focal cerebral ischemia in rats. Methods The rat model of middle cerebral artery ischemia was established using suture-occluded method. The pathological morpholo? gy of brain tissue was examined by using HE staining. The expression levels of GAP-43 SYN, nutrient factor (BDNF VEGF Ang1) and inhibitory factor (NogoA NogoR RhoA) were determined by using Western blotting technique. Results The number of neurons in ischemic penumbra was significantly decreased in model group (P<0.01) than in sham-operat? ed group. The expression levels of GAP-43 were significantly decreased (P<0.05) while the expression levels of NogoA NgR and RhoA in the thalamus were significantly increased (P<0.05) in model group than in sham-operated group. The expression levels of SYN and nutrient factors (BDNF VEGF Ang1) were not different between the model group and sham-operated group. Conclusion The increase in nerve inhibitory factors may contribute to the down-regulation of neu?rogenesis at ischemic neocortical penumbra after focal cerebral ischemia in rats.
