Detetmination of Syringin, Ferulic Acid and Salvianolic Acid B in Huoluo Xiaotong Tablets by HPLC
- VernacularTitle:HPLC法同时测定活络消痛片中紫丁香苷、阿魏酸和丹酚酸 B含量
- Author:
Bing HU
;
Dingfeng WANG
- Publication Type:Journal Article
- Keywords:
Huoluo Xiaotong tablets;
Syringin;
Ferulic acid;
Salvianolic acid B;
HPLC
- From:
China Pharmacist
2015;(2):315-316,357
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a determination method for syringin, ferulic acid and salvianolic acid B in Huoluo Xiaotong tabletsbyHPLC.Methods:ThecolumnwasKromasilC18(150mm×4.6mm,5 μm),themobilephasewasmethanol-0.2% phos-phoric acid solution with gradient elution at the flow rate of 1. 0 ml·min-1 , the detection wavelengths were 265nm for Sgnigin, 321nm for Ferulic acid and 286nm for Salvianolic acid B. The column temperature was 25℃. Results: Syringin, ferulic acid and salvianolic acid B showed good linear relationship within the range of 64. 500-1. 032 × 103 , 7. 525-120. 400, 65. 250-1. 044 × 103 μg·ml-1 with the average recovery of 100. 75%, 99. 56% and 99. 75%, respectively. Conclusion:The method is simple, accurate and rapid.
