Induced CD4~+ CD25~+T lymphocytes in rhG-CSF mobilized peripheral blood by TGF-?_1
- VernacularTitle:应用TGF-?_1从rhG-CSF动员的外周血采集物中诱导产生CD4~+ CD25~+调节性T细胞
- Author:
Zhimin SHANGGUAN
;
Baolong WANG
;
Xiaoliu LIANG
- Publication Type:Journal Article
- Keywords:
TGF-?1;
G-CSF;
Peripheral blood stem cell graft;
CD4+CD25+ regulatory T lymphocyte
- From:
Chinese Journal of Blood Transfusion
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To induce CD4+CD25+ regulatory T lymphocytes from mononuclear cells of rhG-CSF mobilized peripheral blood,and to analyze the phenotype and function of them.Methods Blood samples of ten donors of peripheral blood stem cell were collected,CD4+CD25–T lymphocytes from rhG-CSF mobilized peripheral blood(G-PB group) and unmobilezed peripheral blood(PB group) were isolated with a CD4+CD25+T lymphocytes selection kit,and then were induced by TGF-?1.FCM,RT-PCR,cell proliferration and suppression assay were used to test the expression of CD25+,Foxp3 and suppressive function,and the difference of the rate and the suppressive activity of CD4+CD25+T cells were compared.Results 1) After treated with anti-CD3mAb and TGF-?1,there was a difference between CD4+CD25–T lymphocytes isolated from G-PB group and PB group,the expression of CD25+ were(77.9?2.3)% and(65.7?4.2)%,respectively(P
